茄枝视频The U.S. has long opposed the 750-mile pipeline, arguing it will leave Europe overly dependent on Russia for energy supply and extend President Vladimir Putin's influence over the continent.
Trump said last year that "Germany is a captive of Russia," in an apparent reference to the pipeline. The comments caused a stir at the start of a tense NATO summit.
Friday's legislation threatening U.S. sanctions had an immediate impact, with a company that operates ships laying sections of the undersea pipeline saying Saturday it had suspended activities relating to the project “in anticipation of the enactment of the National Defense Authorization Act (NDAA)."
Switzerland-based Allseas said it will seek “guidance comprising of the necessary regulatory, technical and environmental clarifications from the relevant U.S. authority.”
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茄枝视频However those behind the project insisted it would go ahead in spite of the U.S. efforts.
茄枝视频Construction of the pipeline is already well advanced, and it wasn't immediately clear what the impact will be.
"Completing the project is essential for European supply security," said Nord Stream 2 spokesman Jens Mueller in a statement.
"We together with the companies supporting the project will work on finishing the pipeline as soon as possible."
Russia provides about 37 percent of Europe's natural gas, and currently, the bulk of it moves through Ukraine's pipelines.
The Nord Stream 2 project would double the amount of gas Russia can send directly to Germany and allow it to bypass Eastern European nations like Poland and Ukraine.
茄枝视频But Russia doesn't have the capability to install undersea pipelines and is relying on the technological expertise of European companies.
茄枝视频Germany has been vehemently opposed to the sanctions but was unsuccessful lobbying against them.
茄枝视频“The German government rejects such extraterritorial sanctions,” spokeswoman Ulrike Demmer said in a statement.
“They affect German and European companies and constitute an interference in our domestic affairs.”
While the sanctions pose problems for some, Ukraine hoped it could be a game changer by giving Kyiv more leverage in negotiations with Moscow over the future use of its pipelines.
In an interview with NBC News last week, the CEO of Ukraine's state-owned energy company Naftogaz, 茄枝视频Andriy Kobolyev, said the sanctions would have a major impact on the stalled negotiations between Russia and Ukraine over the transport of natural gas to Europe.
"I believe the European gas market will benefit significantly, and Ukraine will benefit," he said.
茄枝视频On Saturday, the two countries' gas operators reached a new five-year deal in Vienna that would see Moscow pay $2.9 billion to Kyiv to maintain access to their pipelines. In exchange, Ukraine would drop outstanding legal claims against Russia by Dec. 29.
茄枝视频The transmission tariff for Russian gas will increase, however, Ukraine's energy minister said.
Linda Givetash is a reporter based in London. She previously worked for The Canadian Press in Vancouver and Nation Media in Uganda.
Dan De Luce, Associated Press and Reuters contributed.